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Peer-reviewed veterinary case report

Differentiating blood samples from scrapie infected and non-infected hamsters by detecting disease-associated prion proteins using Multimer Detection System.

Journal:
Biochemical and biophysical research communications
Year:
2010
Authors:
An, S S A et al.
Affiliation:
Department of Bionano Technology · South Korea
Species:
rodent

Abstract

This communication describes the application of a modified sandwich enzyme-linked immunosorbent assay (ELISA), termed Multimer Detection System (MDS) for the detection of disease-associated multimeric forms of the prion protein (PrPd) in hamster blood. PrPd was detected in plasma of prion-affected hamsters while MDS revealed no PrPd in identically-treated plasma of healthy animals. This is the first report of a single ELISA- based immune detection of PrPd from blood samples.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/20085753/