Peer-reviewed veterinary case report
Differentiation of infectious bronchitis virus vaccine strains Ma5 and 4/91 by TaqMan real-time PCR.
- Journal:
- Polish journal of veterinary sciences
- Year:
- 2017
- Authors:
- Stenzel, T et al.
- Affiliation:
- .
Abstract
The aim of this study was to develop rapid molecular assays for differentiating vaccine strains Ma5 and 4/91 of the infectious bronchitis virus (IBV). Specific primers and probes for S1 and N genes were designed based on the nucleotide sequences of both vaccine strains. Cross-reactivity was not observed. Assay sensitivity was 2.373 × 103 copies of the Ma5 strain, and 3.852 x 103 copies of the 4/91 strain. Samples belonging to a known genotype demonstrated that the designed assays supported rapid and sensitive detection of Ma5 and 4/91 vaccine strains of IBV.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/29166280/