Peer-reviewed veterinary case report
Direct culture-independent sequence typing ofobtained from genital swabs and frozen semen samples from South African horses.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2019
- Authors:
- May, Catherine E et al.
- Affiliation:
- Department of Production Animal Studies (May
- Species:
- horse
Abstract
We report herein the use of crude extracts obtained from samples of-infected horses for the purpose of multi-locus sequence typing (MLST). Samples (= 36) were collected from horses in South Africa from 1996 to 2017: 34 from genital swabs (stored at -20°C for 2-3 y) and 2 from cryopreserved raw semen aliquots (stored at -70°C for 18 y) prior to assay. The MLST assay showed a single sequence type (ST), designated ST4, that supported a point introduction and thus a common source for the South African outbreak of contagious equine metritis.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/31423914/