Peer-reviewed veterinary case report
Rapid test to identify parvovirus types in dog poop samples
By V, Pavana Jyothi et al.·Published in Journal of virological methods·2016·Translational Research Platform for Veterinary Biologicals, India·View original on PubMed →
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Original publication title: Direct typing of Canine parvovirus (CPV) from infected dog faeces by rapid mini sequencing technique.
- Species:
- dog
Plain-English summary
A group of 84 dogs showing symptoms of enteritis, like vomiting and diarrhea, were tested for canine parvovirus (CPV) using a new rapid mini-sequencing technique. Out of these, 76 dogs tested positive for CPV, with 74 identified as type 2a and 2 as type 2b. This new method proved to be very effective, accurately typing the virus even in samples with low levels of the virus, which traditional methods struggled with. The researchers recommend this mini-sequencing technique for quick and reliable identification of CPV types in dogs.
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Abstract
Canine parvovirus (CPV) is a non-enveloped single stranded DNA virus with an icosahedral capsid. Mini-sequencing based CPV typing was developed earlier to detect and differentiate all the CPV types and FPV in a single reaction. This technique was further evaluated in the present study by performing the mini-sequencing directly from fecal samples which avoided tedious virus isolation steps by cell culture system. Fecal swab samples were collected from 84 dogs with enteritis symptoms, suggestive of parvoviral infection from different locations across India. Seventy six of these samples were positive by PCR; the subsequent mini-sequencing reaction typed 74 of them as type 2a virus, and 2 samples as type 2b. Additionally, 25 of the positive samples were typed by cycle sequencing of PCR products. Direct CPV typing from fecal samples using mini-sequencing showed 100% correlation with CPV typing by cycle sequencing. Moreover, CPV typing was achieved by mini-sequencing even with faintly positive PCR amplicons which was not possible by cycle sequencing. Therefore, the mini-sequencing technique is recommended for regular epidemiological follow up of CPV types, since the technique is rapid, highly sensitive and high capacity method for CPV typing.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/27659245/