Discovering the Potent Inhibitors Againstandby Repurposing the Natural Product Compounds.

Abstract

In the present study, we screened 502 natural product compounds against thegrowth of(.). Then, the novel and potent identified compounds were further evaluated for theirefficacies using viability and cytotoxicity assays. Theinhibitory effects of the selected compounds were evaluated using"rodent strain" in mice model. Three potent compounds, namely, Rottlerin (RL), Narasin (NR), Lasalocid acid (LA), exhibited the lowest IC(half-maximal inhibitory concentration) as follows: 5.45 ± 1.20 μM for RL, 1.86 ± 0.66 μM for NR, and 3.56 ± 1.41 μM for LA. The viability result revealed the ability of RL and LA to prevent the regrowth of treated parasite at 4 × ICand 2 × IC, respectively, while 4 × ICof NR was sufficient to stop the regrowth of parasite. The hematology parameters ofwere different in the NR-treated groups as compared to the infected/untreated group. Interestingly, intraperitoneal administration of NR exhibiting inhibition in the growth ofin mice was similar to that observed after administration of the commonly used antibabesial drug, diminazene aceturate (DA) (76.57% for DA, 74.73% for NR). Our findings indicate the richness of natural product compounds by novel potent antibabesial candidates, and the identified potent compounds, especially NR, might be used for the treatment of animal babesiosis.