discovery utilizing indirect-ELISA in East Java, Indonesia as a new approach in detecting leucocytozoonosis.

Abstract

BACKGROUND: Leucocytozoonosis, caused by, is a significant parasitic disease in poultry and can cause substantial economic losses in the broiler chicken industry while the golden standard of examination is currently absent. AIM: This study aimed to detect the presence ofin broiler chickens in East Java Province using two different diagnostic methods. METHODS: Blood samples were taken from the brachial vein of death broiler chickens from several farms recorded as endemic areas of leucocytozoonosis in East Java, Indonesia. Parasite detection was performed by parasitological examination (blood smear) and ELISA serological examination. RESULTS: The results showed a prevalence ofwas 13.43% based on blood smear examination, whereas ELISA serological examination showed a higher prevalence (27.78%). This difference in results indicates that serological tests have a higher sensitivity in detectinginfections than parasitological methods. CONCLUSION: These findings emphasize the importance of using appropriate diagnostic methods to control leucocytozoonosis in broiler chicken farms. Furthermore, the results of this study can serve as a basis for developing more effective control strategies against this disease in East Java Province.