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Peer-reviewed veterinary case report

Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene.

Journal:
Parasitology research
Year:
2004
Authors:
Spitalska, Eva et al.
Affiliation:
School of Agriculture · United Kingdom

Abstract

Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/15372232/