Disrupted Neuronal Dynamics of Reward Encoding in the Medial Prefrontal Cortex and the Ventral Tegmental Area after Episodic Social Stress.

Abstract

Previous research suggests that stress predisposes individuals to develop substance use disorders by disrupting the brain processing of rewards. Yet, how stressful experiences disrupt the brain processing of reward-related cues at the neuronal level is poorly understood. Intermittent social defeat (ISD) is a stress animal model that increases reward-seeking behavior, drug self-administration, and choice impulsivity up to several weeks after stress. We tested the hypothesis that ISD disrupts the neuronal encoding of reward cues in key areas of the brain that regulate reward-seeking. We examined in vivo neuronal dynamics in response to reward cues in the dorsal medial prefrontal cortex (dmPFC) and the ventral tegmental area (VTA) simultaneously, and longitudinally, in control and stressed Long-Evans male rats during a discriminative stimulus reward-seeking task. In the dmPFC, ISD decreased cue-evoked neuronal activity 1 and 15 d after stress, which indicates a long-term degradation of outcome anticipation-related processing. In the VTA, ISD increased cue-evoked neuronal activity 1 d after stress but decreased cue-evoked activity 15 d after stress. Moreover, decoding analysis in single neurons and populations showed parallel increases and decreases in reward discrimination accuracy in the VTA which points to time-dependent changes in incentive salience after stress. These results demonstrate that ISD differently disrupts the neuronal encoding of reward cues in the dmPFC and the VTA and identify novel neurofunctional signatures that underlie a higher predisposition to seek out rewards after stress.