Distribution, identification and ecology of <i>Phortica</i> genus (Diptera: Drosophilidae) in Spain.

Abstract

The genus <i>Phortica</i> (Diptera: Drosophilidae) includes five species of small flies in Europe. <i>Phortica variegata</i>, the zoophilic fruit fly, is the main vector of <i>Thelazia callipaeda</i>, a zoonotic parasite that is rapidly spreading througout Europe. Despite extensive studies on thelaziosis in animals and humans, there is limited knowledge about the geographical distribution and hovering activity of these vector flies. In 2023, 1,462 <i>Phortica</i> flies were sampled across 12 Spanish provinces, providing new records of <i>Phortica variegata</i> and <i>Phortica oldenbergi</i>. Surprisingly, <i>P. oldenbergi</i>, previously considered a rare Afrotropical species, was prevalent in most regions sampled in Spain. However, <i>Phortica semivirgo</i> was not collected. The abundance of <i>Phortica</i> spp. correlated positively with altitude and certain tree species. Rural oak-wooded areas in central and northern Spain showed the highest densities of <i>P. variegata</i>. Both drosophilid species were analysed morphologically and molecularly, providing new morphological descriptors and sequence barcodes for species identification. Phylogenetic analysis based on COI sequences, showed <i>P. oldenbergi</i> grouped with Asian origin <i>Phortica</i> species, while <i>P. variegata</i> in America was closer to Spanish sequences than those from other European countries. The hovering activity of <i>P. variegata</i> causes significant discomfort to humans during outdoor activities. This paper also reviews the historic records of <i>P. variegata, P. semivirgo</i> and <i>P. oldenbergi</i> in Spain over the last 90 years. This study enhances the understanding of the distribution, identification, ecology, and behaviour of these zoophilic flies in Europe.