Peer-reviewed veterinary case report
Test to detect Echinococcus granulosus infection in dogs using
By Benito, Aitziber & Carmena, David·Published in Acta tropica·2005·Department of Immunology, Spain·View original on PubMed →
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Original publication title: Double-antibody sandwich ELISA using biotinylated antibodies for the detection of Echinococcus granulosus coproantigens in dogs.
- Species:
- dog
Plain-English summary
A study tested a new blood test to detect Echinococcus granulosus, a parasite that can infect dogs and cause serious health issues. The test was able to identify the parasite in fecal samples from infected dogs with a good level of accuracy, showing a sensitivity of 78.4% and a specificity of 93.3%. This means it can effectively help veterinarians diagnose infections and monitor the health of dogs. The researchers believe this test could be useful for controlling and preventing this type of intestinal infection in dogs.
People also search for: dog parasite test · Echinococcus granulosus symptoms in dogs · how to treat dog intestinal parasites
Abstract
Here we present the diagnostic evaluation of an improved double-antibody sandwich ELISA for detecting Echinococcus granulosus antigens in dog faecal samples (coproantigens). A purified rabbit IgG fraction against protoscolex excretory-secretory products was used as primary antibody, and the same fraction conjugated with biotin as secondary antibody. In order to validate the sandwich ELISA, intra- and inter-assay precision, linearity, and recovery percentages were calculated. The diagnostic evaluation of the method was carried out by investigating faecal samples from 37 dogs naturally infected with E. granulosus, 15 Echinococcus-free dogs infected with Taenia spp., 82 dogs with non-taeniid helminths and 66 dogs free of helminth infections. An overall sensitivity of 78.4% and specificity of 93.3% were determined. Positive and negative predictive values were 72 and 95%, respectively, and the diagnostic efficiency was 90.5%. In addition, the sandwich ELISA detection limit was estimated in 5.12 ng ml(-1). These results are highly satisfactory, allowing the use of this methodology in surveillance and control programs for intestinal echinococcosis in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/15863093/