Dynamics of Endogenous Somatostatin and the Infarct-Limiting Effect of Octreotide in a Rat Model of Long-Term Ischemia/Reperfusion.

Abstract

Outbred male Wistar rats underwent coronary artery occlusion (45 min) followed by reperfusion (120 min), and the infarct size to area at risk (IS/AAR) ratio was determined. For 8 days prior to coronary occlusion, rats were subcutaneously administered octreotide at doses of 20 and 40 μg/kg. In the control group rats, the levels of endogenous somatostatin were measured before occlusion and at the 120th minute of reperfusion. Preventive administration of 40 μg/kg octreotide reduced the levels of creatine kinase-MB (CK-MB) and infarct size by decreasing the necrosis area. Octreotide at a dose of 20 μg/kg produced no infarct-limiting effect. Control animals exhibited increased endogenous somatostatin levels by the end of reperfusion (in comparison with baseline), which negatively correlated with CK-MB levels.