Early postnatal protein malnutrition causes resistance to the anxiolytic effects of diazepam as assessed by the fear-potentiated startle test.

Abstract

Given that protein malnutrition induces structural, neurochemical and functional changes in the CNS, the present study aimed to investigate the effects of different early periods of protein malnutrition on the behavior and reactivity to diazepam (DZ) in a model of anxiety: the fear-potentiated startle (FPS). Male Wistar rats (n = 110) from well-nourished (16 %-protein) or malnourished (6%-protein) litters were distributed in five different groups: W (well-nourished), M7 (malnourished for 7-days, since day 0), M14 (14-days), M21 (21-days) and M28 (28-days). The results obtained in FPS revealed that malnourished-animals acquired the startle response, irrespective of the time they were exposed to the diet. Besides, DZ reduced the startle amplitude in the noise-alone and light-noise trials. The data concerning the total freezing time showed that the expression of this response was affected by malnutrition and varied in accordance with the findings of previous studies in which malnutrition procedures was imposed for long periods (more than 50 days). Therefore, we suggest that early protein malnutrition: (a) did not produce deficits in the associative learning process of these animals in the FPS, and (b) decreased freezing time in the FPS and produce hyporeactivity to the effects of DZ in rats malnourished for 21 days or more, indicating alterations in the GABAergic neurotransmitter system.