Effect of combined infection withand influenza virus on their respective proliferation in chicken embryonated eggs.

Abstract

BACKGROUND: is a major food-borne bacterial pathogen that causes food poisoning related to the consumption of eggs, milk, and meat. Food safety in relation tois particularly important for eggs because their shells as well as their contents can be a source of contamination. Chicken can also be infected with influenza virus, but it remains unclear how co-infection ofand influenza virus affect each other. AIM: The potential influence of co-infection ofand influenza virus was examined. METHODS: Abony and influenza virus were injected into chicken embryonated eggs. After incubation, proliferation ofand influenza virus was measured using a direct culture assay for bacteria and an enzyme-linked immunosorbent assay for influenza virus, respectively. RESULTS: Our findings indicate that the number of colony-forming units (CFUs) ofdid not vary between chicken embryonated eggs co-infected with influenza A virus and-only infected eggs. Furthermore, we found the proliferation of influenza A or B virus was not significantly influenced by co-infection of the eggs with. CONCLUSION: These results suggest that combined infection ofwith influenza virus does not affect each other, at least in terms of their proliferation.