Peer-reviewed veterinary case report
Effect of Cryopreservation on Sperms Function Parameters and In vitro Fertilization Rate in Mice
- Journal:
- The Iraqi Journal of Veterinary Medicine
- Year:
- 2013
- Authors:
- Ilaf Hassan Hadi
- Affiliation:
- Department of Anatomy Histology and Embryology, College of Veterinary Medicine, Baghdad University, Iraq · IQ
- Species:
- rodent
Abstract
The study was carried out to investigate the effect of cryopreservation in liquid nitrogen (- 196ºC) on sperm quality (concentration, motility, morphology) and in vitro fertilization rate (IVF), 24 hours post insemination in mice. The sperms were obtained from caudal epididymis of 20 mature male mice and prepared by Direct Activation Technique after cryopreservation followed by insemination the oocyte of 20 female mice using in vitro fertilization procedure. Results showed that there were significant (P > 0.05) decrease in sperms concentration, motility and morphology post cryopreservation. In addition, the results demonstrated that cryopreservation significantly decreased (P > 0.05) fertilization rate in treated mice (36%) compared to control mice (56%). It is conclusion that frozen sperm can be used without compromising pregnancy chances.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →