Effect of experimentally induced endotoxaemia and meloxicam administration on the haemostatic system in donkeys.

Abstract

BACKGROUND: Endotoxaemia is a common condition in equids, frequently accompanied by alterations in haemostasis. Non-steroidal anti-inflammatory drugs, such as meloxicam, have been proven to alleviate some signs of endotoxaemia in donkeys. Neither the haemostatic response to induced endotoxaemia nor the effect of meloxicam in this regard have been described in donkeys. OBJECTIVES: (a) To characterise the haemostatic changes in response to induced endotoxaemia in healthy donkeys, and (b) to assess the effect of meloxicam in these animals. STUDY DESIGN: In vivo experiments. METHODS: Endotoxaemia was induced by LPS (20 ng/kg) infusion in six healthy adult Andalusian donkeys. All animals randomly received either an IV bolus of saline solution (untreated group) or meloxicam (0.6 mg/kg) after LPS infusion (PLI), with a 1-month washout period between trials. Samples were collected at -30, 0, 30, 60, 90, 120, 180 and 360 min PLI for clotting times, antithrombin III, anti-Xa activities, platelet and fibrinogen concentrations; and at -30, 90, 180 and 360 min PLI for viscoelastography (Sonoclot). RESULTS: LPS caused prothrombotic-specific viscoelastographic changes (short activated clot time and elongated clot rate formation) and lower antithrombin III activities compared with reference range for this species. Prolonged prothrombin times were also seen at late timepoints in the experiment. Meloxicam administration resulted in no significant differences between groups. MAIN LIMITATIONS: Short sampling duration, only Andalusian donkeys. CONCLUSIONS: Acute endotoxaemia induced hypercoagulability and initial signs of a consumptive coagulopathy in donkeys. Despite a more prolonged increase in anti-Xa activity, meloxicam did not ameliorate most of these haemostatic changes.