Peer-reviewed veterinary case report
Effect of extrusion processing on immune activation properties of hazelnut protein in a mouse model.
- Journal:
- International journal of food sciences and nutrition
- Year:
- 2016
- Authors:
- Ortiz, Tina et al.
- Affiliation:
- Department of Food Science and Human Nutrition · United States
- Species:
- rodent
Abstract
Although food processing can alter food allergenicity, the impact of extrusion processing on in vivo hazelnut allergenicity is unknown. Here, we tested the hypothesis that extrusion processing will alter the immune activation properties of hazelnut protein (HNP) in mice. Soluble extrusion-processed HNP (EHNP) was prepared and evaluated for immune response using an established transdermal sensitization mouse model. Mice were sensitized with identical amounts of EHNP versus raw HNP. After confirming systemic IgE, IgG1 and IgG2a antibody responses, oral hypersensitivity reaction was quantified by hypothermia shock response (HSR). Mechanism was studied by measuring mucosal mast cell (MMC) degranulation. Compared to raw HNP, the EHNP elicited slower but similar IgE antibody (Ab) response, lower IgG1 but higher IgG2a Ab response. The EHNP exhibited significantly lower oral HSR as well as MMC degranulation capacity. These results demonstrate that the extrusion technology can be used to produce soluble HNP with altered immune activation properties.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/27251648/