Effect of infusion solutions on circulating blood volume through different administration routes in animal models of peritonitis.

Abstract

OBJECTIVE: Aim: To compare the effects of different administration routes of various infusion solutions on the dynamics of circulating blood volume (CBV) in an experimental rat model of peritonitis. PATIENTS AND METHODS: Materials and Methods: The experiment involved 100 male white rats, three months old, weighing 200-220 g, born to different dams. Peritonitis was modeled by a single intraperitoneal injection of a 10% suspension of fresh rat feces. During the study, following the development of peritonitis in the animals, the dynamics of neutral red dye concentration in the gastric perfusate were measured. This was done using 0.9% sodium chloride solution and 10% albumin solution, administered via three different routes. RESULTS: Results: It was determined that intraosseous administration of both 0.9% NaCl solution and 10% albumin solution had a statistically significant impact on CBV in peritonitis. The 10% albumin solution demonstrated a longer hemodynamic effect. Additionally, extraperitoneal administration of the 10% albumin solution was also effective. CONCLUSION: Conclusions: 1. Administration of 0.9% sodium chloride and 10% albumin solutions in experimental peritonitis in animals results in an increase in circulating blood volume (CBV) when administered by the intraosseous route, with increases of 30% (Р≤0.01) and 45% (Р≤0.001), respectively. 2. The 10% albumin solution, administered extraperitoneally as a bolus infusion, significantly enhanced the secretion of neutral red dye into the perfusate, which correlated with its effects on CBV and improved microcirculation in the gastric mucosa.