Peer-reviewed veterinary case report
How inflammation affects gut lining in dogs with IBD
By Ogawa, Misato et al.·Published in Journal of veterinary internal medicine·2018·Cooperative Department of Veterinary Medicine, Japan·View original on PubMed →
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Original publication title: Effect of interleukin-1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease.
- Species:
- dog
Plain-English summary
A group of dogs with inflammatory bowel disease (IBD) showed signs of intestinal inflammation, which can cause symptoms like vomiting, diarrhea, or weight loss. Researchers found that a protein called interleukin-1 beta (IL-1β) was present in higher amounts in the colons of these dogs compared to healthy dogs, and this protein seemed to reduce the expression of a key component that helps maintain the intestinal barrier. This suggests that the imbalance of IL-1β may contribute to the ongoing inflammation and intestinal issues in dogs with IBD. Understanding this could help veterinarians develop better treatments for managing IBD in dogs.
People also search for: dog inflammatory bowel disease symptoms · dog diarrhea treatment · IBD in dogs management
Abstract
BACKGROUND: Mucosal imbalance of interleukin (IL)-1β and IL-1 receptor antagonist (Ra) has been reported in the duodenal mucosa of dogs with inflammatory bowel disease (IBD). However, the imbalance in the colonic mucosa and its role in duodenitis and colitis in IBD of dogs remain unclear. OBJECTIVES: To measure the expression of IL-1β and IL-1Ra proteins in the colonic mucosa of dogs with IBD, and to determine the effect of IL-1β on expression of occludin (ocln) mRNA, a tight junction component, in the duodenal and colonic mucosa of dogs with IBD. ANIMALS: Twelve dogs with IBD and 6 healthy dogs. METHODS: IL-1β and IL-1 Ra proteins in the colonic mucosa were quantified by ELISA in 7 of the 12 dogs with IBD. Expression of ocln mRNA in the duodenal and colonic mucosa was examined in the 12 dogs by real-time PCR. RESULTS: The ratio of IL-1β to IL-1Ra in the colonic mucosa was significantly higher in dogs with IBD than in healthy dogs. The ex vivo experiment determined that IL-1β suppressed expression of ocln mRNA in the colonic mucosa, but not in the duodenal mucosa, of healthy dogs. Expression of ocln mRNA in the colonic mucosa, but not in the duodenal mucosa, was significantly lower in dogs with IBD than in healthy dogs. CONCLUSIONS AND CLINICAL IMPORTANCE: A relative increase in IL-1β may attenuate ocln expression, leading to intestinal barrier dysfunction and promotion of intestinal inflammation in the colonic mucosa, but not in the duodenal mucosa, of dogs with IBD.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/29572935/