Effect of kelp powder on the resistance of Aeromonas hydrophila in the gut of hybrid snakeheads (Channa maculata ♀ × Channa argus ♂).

Abstract

To assess the level of oxidative stress, expression of immune-related genes, histomorphology, and changes in the intestinal tract of hybrid snakeheads(Channa maculata ♀ × Channa argus ♂) under stress from kelp powder in place of flour against Aeromonas hydrophila. We set up experimental diets: a control (C) diet of 20% flour, an experimental (MR) diet of 10% kelp powder and 10% flour, and an experimental (FR) diet of 0% starch and 15% kelp powder. The experimental fish in each group were infected with Aeromonas hydrophila after 60 days of feeding. For this experiment, some of the experimental fish in group C were injected with PBS as a negative control group (PBS). The results showed that the C group had significantly higher SOD, CAT, and T-AOC activity and expression of TAK1, IKKβ, IL-1β, and TNF-α genes in the MyD88 pathway than the PBS group. CAT activity and the expression of TAK1, IL-1β and TNF-α genes in the MyD88 pathway were significantly lower in the MR group than in the C group. Furthermore, the number of goblet cells in the MR group was significantly higher than in the C group. Furthermore, microorganisms such as Bacteroidota and Actinobacteriota were significantly lower in the C group than in the PBS and FR groups, as were beneficial bacteria such as Clostridium_sensu_stricto_1 and Sphingomonas. Replacing flour with kelp powder increases hybrid snakehead gut resistance to Aeromonas hydrophila.