Ozonized saline effects on oxidative stress in cats with FIP

Abstract

Feline infectious peritonitis is a viral disease in cats characterized by systemic involvement and a frequently fatal outcome. Diagnosis is established through a comprehensive assessment of clinical signs and laboratory findings. The aim of this study was to evaluate the efficacy of a single intravenous administration of 100 mL of 0.9 % isotonic sodium chloride solution, ozonated at a concentration of 5 μg/mL for 5 minutes using the Vetozone Medical Ozone Device, on oxidative stress parameters and survival time. In this context, oxidative stress markers were compared  before  and  after  treatment to provide scientific data on the potential therapeutic effects of ozonated saline in veterinary medicine. The study was conducted on a sample of 10 domestic cats diagnosed with Feline infectious peritonitis, all of which were monitored for a follow-up period of 6 months to assess clinical progression and survival. In the study conducted on cats diagnosed with Feline infectious peritonitis, administration of ozonated saline resulted in increased total antioxidant capacity and native thiol levels, accompanied by decreases in total oxidant capacity, oxidative stress index, and disulfide levels at the 1-hour mark. Although these changes were not statistically significant (P> 0.05), the findings suggest that ozone exerts a regulatory effect on oxidative stress and helps restore the thiol–disulfide balance toward an antioxidant state. In addition, the treatment markedly improved clinical signs, reduced oxidative stress markers, and achieved a 100 % survival rate in the treated cats. These results indicate that ozonated saline solution, by reducing free radical–induced cellular damage in cats with Feline infectious peritonitis, has the potential to be used as a supportive (adjuvant) approach rather than as a directly curative therapeutic agent. Nevertheless, larger controlled clinical studies with extended follow-up periods are required to validate these effects and to standardize the therapeutic protocol.