Effect of Platelet-Rich Fibrin on Peripheral Nerve Regeneration.

Abstract

PURPOSE: This study aimed to evaluate the effect of platelet-rich fibrin (PRF) on peripheral nerve regeneration on the sciatic nerve of rats by using functional, histopathologic, and electrophysiologic analyses. MATERIALS AND METHODS: Thirty female Wistar rats were divided randomly into 3 experimental groups. In group 1 (G1), which was the control group, the sciatic nerve was transected and sutured (n&#x200a;=&#x200a;10). In group 2 (G2), the sciatic nerve was transected, sutured, and then covered with PRF as a membrane (n&#x200a;=&#x200a;10). In group 3 (G3), the sciatic nerve was transected, sutured by leaving a 5-mm gap, and then covered by PRF as a nerve guide (n&#x200a;=&#x200a;10). Functional, histopathologic, and electrophysiologic analyses were performed. RESULTS: The total histopathologic semiquantitative score was significantly higher in G1 compared to G2 and G3 (P&#x200a;<&#x200a;0.05). Myelin thickness and capillaries were significantly lower in G3 compared to G1 (P&#x200a;<&#x200a;0.05). There was no statistically significant difference between the groups with regard to the functional and electrophysiologic results. CONCLUSION: The study results suggest that PRF decreases functional recovery in sciatic nerve injury. Further studies are required to determine the efficacy of PRF on peripheral nerve regeneration.