Effect of transport stress on apoptosis and autophagy in goat lung cells.

Abstract

INTRODUCTION: Road transportation exposes goats to thermal, mechanical, and microbial stressors that can compromise their welfare by triggering pulmonary apoptosis and autophagy processes associated with tissue damage and immunosuppression. METHODS: To explore potential biomarkers for transport-related welfare assessment, this study analyzed lung tissues from nine Ganxi goats (= 9; 0 h control, 2 h/6 h transport groups) through an integrated experimental approach: TUNEL assays quantified apoptosis rates, immunohistochemistry mapped protein localization, Western blotting analyzed protein expression levels, and qPCR profiled gene expression of apoptotic regulators (Bax, Bcl-2) alongside autophagy-related markers (LC3B, p62, PINK1, Parkin). RESULTS: Results indicated time-dependent cellular stress patterns, where the 2 h group displayed elevated apoptosis rates, while the 6 h group exhibited upregulated Parkin expression (< 0.05) and altered regulation of apoptotic [Bcl-2-associated X-protein (Bax)/B-cell lymphoma-2 (Bcl-2)] and autophagy-related genes (Microtubule-associated protein 1 light chain 3B (LC3B), p62, PTEN-induced putative kinase 1 (PINK1)/Parkin). Protein localization analyses revealed compartment-specific responses, with Bcl-2/Bax primarily in bronchial epithelia and LC3B/PINK1/Parkin in alveolar cells, suggesting spatially distinct stress adaptation mechanisms. Observed molecular changes coincided with histological evidence of pulmonary alterations, implying a potential interplay between apoptosis and autophagy in transport-induced cellular stress. The identification of time-sensitive molecular shifts (e.g., transient apoptosis elevation at 2 h, and progressive Parkin activation at 6 h) could inform hypotheses for monitoring transport-associated physiological responses. DISCUSSION: These findings highlight the need for further investigation into transport duration effects, with shorter intervals (e.g., &#x2264;2 h) warranting evaluation for acute stress mitigation, and prolonged transport (e.g., >6 h) requiring characterization of cumulative autophagic impacts. The mechanistic insights can contribute to developing science-informed strategies for assessing transport stress, aligning animal welfare research with objectives to enhance sustainable livestock management practices.