Effect of Tribulus terrestris L. extract in the management of polycystic ovarian syndrome in rat model.

Abstract

OBJECTIVES: Oxidative stress is considered to be a significant factor in the complex disorder known as polycystic ovarian syndrome (PCOS). In this study, we investigated whether using the traditional medicinal plant Tribulus terrestris L. to lower oxidative stress could help alleviate the symptoms of PCOS. METHODS: In this randomized controlled trial, rats were given oral estradiol (4 mg/kg/bw) to induce PCOS, and they were split into four groups: treatment (T. terrestris L. extract, 7 mg/kg/bw), standard control (metformin, 300 mg/kg/bw), positive control, and negative control. Blood and ovarian tissues were taken for histological, hormonal, molecular, and biochemical examinations after 21 days. KEY FINDINGS: Treatment with T. terrestris L. extract resulted in decreased levels of FSH, LH, estrogen, insulin, TOS, and MDA, as well as downregulated expression of IL-6, IL-1, IGF-1, and CYP-19, in comparison to the positive control. Histological examination revealed healthier ovarian morphology with more corpus luteum and fewer cystic follicles, and TAC levels rose. CONCLUSION: The results imply that T. terrestris may help combat hormonal disruption and oxidative stress in PCOS, but human studies are still required for confirmation.