Effects of Chili Straw Biochar on Alfalfa (<i>Medicago sativa</i> L.) Seed Germination and Seedling Growth on Electrolytic Manganese Residue.

Abstract

This study employed a pot experiment to compare the effects of varying application rates of chili straw biochar on seed germination and seedling growth of alfalfa (<i>Medicago sativa</i> L.) cultivated in electrolytic manganese residue (EMR) and to elucidate the underlying mechanisms. We aimed to provide a theoretical basis for vegetation restoration and manganese pollution control at EMR disposal sites. Our results indicated that while chili straw biochar did not affect the seed germination rate, it significantly enhanced the germination energy. In addition, treatment with 5% biochar significantly increased the germination index. Biochar application increased alfalfa seedling height (6.13 cm in the control group vs. 6.63-7.20 cm in the treated groups). Concurrently, the aboveground fresh biomass significantly increased by 49-77% compared to the control. Additionally, biochar application elevated chlorophyll content and reduced malondialdehyde content in alfalfa leaves. Correlation analysis revealed that the primary mechanisms underlying biochar-mediated improvement in seed germination and seedling growth involved enhancing the organic matter, available nitrogen, and available phosphorus content in the EMR, while decreasing the available manganese content. Overall, the application of 5% biochar in EMR optimally improved alfalfa plant growth and development.