Effects of Peripheral Magnetic Stimulation on Bone Healing After Fractures in Mice.

Abstract

Bone fractures, especially in the elderly, are increasing, posing challenges to healthcare systems. Traditional treatment often focuses merely on bone repair and overlooks the overall healing environment. Peripheral magnetic stimulation (PMS), a non-invasive method, shows promise for bone regeneration. In our murine femoral fracture model study, male C57BL6/J mice were divided into a control group and three PMS treatment groups (10, 50, and 100 Hz). After a fracture, the mice received 30-min daily PMS sessions. The pain was monitored weekly using the von Frey test. Micro-computed tomography (μCT), biomechanics, and histology evaluated bone healing. Our results have shown that 10 and 100 Hz PMS significantly reduced pain and promoted early callus formation by speeding up early mineralized callus. After 4 weeks, the 10 Hz PMS improved mechanical strength, and the 10 and 100 Hz PMS increased bone mineral density. Histology revealed more cartilage, new bone formation, and enhanced osteoblast activity. PMS also decreased fibrous tissue, indicating better bone remodeling. The staining results confirmed that PMS promoted early cartilage formation, endochondral ossification, and increased vascular density. These findings suggest that PMS at 10 and 100 Hz accelerates endochondral ossification, enhances bone formation, and improves biomechanical strength, demonstrating its potential application value in fracture treatment.