Efficacy of a probiotic fermented herb in the prevention and treatment of fish nocardiosis.

Abstract

poses a severe threat to the sustainable development in the aquaculture of a virous freshwater as well as marine fish species. In the present study, the herb No.100 (it has not been made public due to patent protection) andMS-2 with obvious inhibitory effects againststrain NS-23 were screened from 190 herbal extracts and 5 probiotic strains for developing probiotic fermented herb (PFH) against nocardiosis in largemouth bass (). An ideal fermentation parameter was established by single-factor optimization as: 4% (w/v) herbal substrate with 4% (v/v) probiotic inoculum at pH 7.5 for 18 h for achieving maximum viable cell count. A 49-day feeding trial with supplements was conducted that the fish with PFH administration were significantly enhanced weight gain rates at 310.51 ± 37.44%, with increasing serum enzyme activities and nitric oxide (NO) levels, up-regulated tissue-specific expression of immune-related genes (,,,,,) as well as mounting up gut microbial diversity (especiallyandspecies) than that of PBS control, herb or probiotic administration. Furthermore, the challenge study withstrain NS-23 was conferred a 77% relative percentage survival (RPS) in the fish with PFH-supplemented diet, and the histopathological analysis confirmed that PFH effectively mitigated multi-organ necrosis and oxidative stress, leading to a complete regression of granulomas in the fish upon artificial challenge. Taken together, this PFH could be served as a safe and effective administrator against fish nocardiosis, offering a sustainable alternative to antibiotics in aquaculture.