Efficacy of Phytopharmaceuticals From the Amazonian Plantfor Wound Healing in Dogs.

Abstract

We comparatively evaluate two distinct formulations containing 5% of Juc&#xe1; () for wound healing in dogs. An excision model study was performed in 11 dogs with three dermal wounds in each animal, which were treated with: (1) topical phytopharmaceutical based on Carbopol (PyC) containing 5% Juc&#xe1; ethanolic extract; (2) topical phytopharmaceutical based onbutter (PyM) containing 5% Juc&#xe1; ethanolic extract; and (3) commercial ointment (control). Wound treatment was carried out on alternated days starting at day (D) one until D21. Macroscopic (all time-points) and histological (D0 and D21) analyses were performed. The antimicrobial activity of Juc&#xe1; was evaluated through Minimal Inhibitory Concentration (MIC). Phytochemical analysis of Juc&#xe1; revealed 3.1% phenolic compound content expressed in rutin and the presence of hydrolyzable tannins and flavonoids. The mean wound retraction was 33.7 &#xb1; 5.5, 34.0 &#xb1; 4.7, and 28.4 &#xb1; 4.9 % for PyC, PyM, and control groups, respectively, with higher wound retraction for both herbal-treated groups compared to the control (< 0.05). Alcoholic extract of Juc&#xe1; had antimicrobial activity against the microorganisms, andat different degrees, with MIC ranging from 250 to 16.625 &#x3bc;g/ml. Microscopic evaluation showed that the phytotherapic formulations contributed to better dermal wound healing through wound fibroplasia. The alcoholic extract of Juc&#xe1; pods has great potential for wound healing in dogs and can be used in the development of commercially viable phytotherapic formulations.