Efficacy of recombinant N- and C-terminal derivative of EmIMP1 againstinfection in chickens.

Abstract

1. Immune mapped protein-1 (IMP1) ofhas been identified as a vaccine antigen candidate forinfection. 2. In the current study, the N- and C-terminal derivative of EmIMP1 were expressed inand administered to chickens. The antibody response, cell-mediated immune (CMI) response and the protective efficacy of the protein vaccines againstchallenge were evaluated. 3. The results showed that C-terminal derivative of EmIMP1 vaccination could increase weight gain, reduce enteric lesions, and decrease faecal oocysts shedding. Moreover, the C-terminal derivative of EmIMP1 caused reasonable improvement in serum antibodies and the numbers of IFN-γ producing peripheral blood mononuclear cells (PBMC), as compared to the control group. 4. This study demonstrated that the C-terminal derivative of EmIMP1 could be used as a potent immunogenic candidate in the development of subunit vaccines againstinfection.