Peer-reviewed veterinary case report
Efficient production of type 2 porcine circovirus-like particles by a recombinant baculovirus.
- Journal:
- Archives of virology
- Year:
- 2008
- Authors:
- Liu, Lan-Jun et al.
- Affiliation:
- Department of Virology II · Japan
Abstract
The capsid protein of PCV2 was expressed by using a recombinant baculovirus with insect Tn5 cells. A large amount of 28-kDa protein was released into the culture medium and self-assembled into PCV2-like particles (PCV2-LPs) with a buoyant density of 1.365 g/cm(3) and a diameter of 20 nm. PCV2-LPs were efficiently expressed, yielding 1 mg of purified particles per 10(7) Tn5 cells. The PCV2-LPs have antigenicity similar to that of authentic PCV2 particles, allowing us to develop a method for sensitively detecting PCV2-specific IgG antibodies. In addition, the PCV2-LPs appeared to be the most promising PCV2 vaccine candidate, by virtue of their potent immunogenicity.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/18998045/