Electron microscopic study of Tetratrichomonas didelphidis and its interaction with a prokaryotic cell.

Abstract

Tetratrichomonas didelphidis is a flagellate protozoan found in the intestine of the opossum. The parasite lives in a hostile and stressed environment, where it interacts with microorganisms and can survive under extreme conditions for growth, involving strict anaerobiosis or equilibration with air and abundance or absence of nutrients. The in vitro cultivation of this protozoan depends upon Escherichia coli as a growth-promoting partner. In this study, we used scanning and transmission electron microscopy to observe the phagocytosis of bacteria by the protozoan, confirming the strong association between both cells and the growth dependence of T. didelphidis upon E. coli. After adherence to the protozoan surface, the bacteria induced the appearance of crater-like depressions and the ingested bacteria were intracellularly degraded.