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Peer-reviewed veterinary case report

Bacteria in infected broken dog teeth studied by gene sequencing

By Rodrigues, Marjory Xavier et al.·Published in Frontiers in veterinary science·2019·Department of Population Medicine and Diagnostic Sciences, United States·View original on PubMed

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Original publication title: Endodontic Microbiome of Fractured Non-vital Teeth in Dogs Determined by 16S rRNA Gene Sequencing.

Species:
dog

Plain-English summary

A study looked at the bacteria found in the teeth of dogs with broken teeth that had exposed pulp, which can lead to infections. Researchers examined samples from ten dogs and found that the bacteria in the infected teeth were diverse and similar to those found in healthy gum areas, but with some differences in types and amounts. This suggests that infections in these teeth may be more complex than previously thought. Understanding this can help veterinarians better diagnose and treat dental infections in dogs.

People also search for: dog broken tooth infection · dog dental bacteria · treatment for dog tooth fracture

Abstract

Dental fractures resulting in pulp exposure will lead to an endodontic infection with microbes from the oral cavity. However, data on the endodontic microbial composition in veterinary dentistry is lacking. The aim of this study was to examine the microbiome of naturally occurring primary endodontic infections in client-owned dogs. The endodontic microbiome of 10 non-vital teeth with exposed pulp cavities was assessed using a 16S rRNA gene sequencing approach. The results were compared to the microbiome of the subgingival plaque of the same teeth. Analysis revealed an abundant mixed microflora of a comparable richness and diversity and with mostly the same phyla obtained from sulcal and endodontic samples. However, further analysis revealed significant differences between sulcal and endodontic samples in the relative abundance of the most abundant phyla and genera, with the relative abundance of Bacteriodetes being significantly higher in endodontic samples. Although each sample presented a particular profile regarding the genera identified,was the most abundant genus in the endodontic samples.was also significantly more abundant in endodontic samples, whileandwere significantly more abundant in sulcal samples. We confirmed that the microbiome of the diseased endodontic system is comparably abundant with microorganisms to the healthy subgingival plaque indicating that previous culture-based studies of primary endodontic infections in dogs underestimated the richness and diversity of the endodontic microbiota.

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Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31649943/