Enhanced Mesophilic Degradation of Rice Straw by Microbial Consortium SXJG15 Through Coordinated Enzymatic Activity and Community Reshaping.

Abstract

The sustainable utilization of rice straw is challenged by its recalcitrant lignocellulosic structure, especially under low-to-moderate field temperatures. In this study, a novel microbial consortium (SXJG15) mainly containing <i>Sphingobacterium</i>, <i>Azospirillum</i>, and <i>Pseudomonas</i> was enriched from overwintering rice stubble in Zhejiang, China, and evaluated for its rice straw degradation efficiency at 25 °C. Over an 18-day cultivation period, SXJG15 achieved a 52.5% degradation of total rice straw, including 60.2% cellulose, 76.3% hemicellulose, and 40.7% lignin. High extracellular enzymatic activities, including cellulases (up to 80.3 U/mL) and xylanases (up to 324.8 U/mL), were observed during the biodegradation process. <i>16S rRNA</i> gene sequencing and metagenomics analyses revealed a succession of dominant taxa, including <i>Sphingobacterium</i>, <i>Azospirillum</i>, and <i>Cellulomonas</i>. Further, CAZy annotation indicated that the SXJG15 enzyme system was rich in glycoside hydrolases (42.7%) and glycosyltransferases (34.2%), demonstrating its high potential for lignocellulose degradation. This study uniquely demonstrates the mesophilic (moderate temperature 25 °C) efficiency of SXJG15 in lignocellulose breakdown, provides new insights into the microbial mechanisms of straw decomposition, and lays a foundation for bioenergy and soil fertility applications for developing a sustainable agriculture system.