Enhanced precision in intracerebral hemorrhage modeling via methodological improvements and outcomes.

Abstract

Previous studies have shown that the production of a simple, stable and reproducible animal model has become indispensable for the study of intracerebral hemorrhage. In this study, a mouse model of intracerebral hemorrhage was constructed via the type VII collagenase injection method, which was improved for precise positioning and injection, thereby accelerating the experimental process and increasing the success rate of this model, providing a strong experimental basis for subsequent research into the pathogenesis and treatment of intracerebral hemorrhage. Type VII collagenase was injected via a microinjector with a very fine diameter glass microelectrode into the right caudate-putamen of C57BL/6 male mice at a constant rate via an electronic microinjector with a constant-flow pump under a digital brain stereotactic apparatus, and neurobehavioral changes and hematoma formation were examined to confirm the success of the intracerebral hemorrhage model. The neurobehavioral scores and hematoma diameter of the mice increased after surgery, and the left limb was hemiplegic, with the most severe symptoms at 24&#xa0;h (P&#x2009;<&#x2009;0.05). The improved methodology presents a specific region of the caudate-putamen with a stable and controllable hematoma size. Gross morphological observations revealed that the change in hematoma diameter was consistent with the change in symptoms of neurological deficits in the mice (P&#x2009;<&#x2009;0.05). Preclinical models of intracerebral hemorrhage established by improved methods can serve as effective models for the study of its pathophysiology and therapeutics.