Enhancement of accuracy and precision of cell Young's modulus measurement: FEM optimization and algebraic compensation.

Abstract

This study addresses the inaccuracy of the Hertz model in measuring single-cell Young's modulus via probe-based indentation. We propose two complementary correction strategies: finite element model optimization and an algebraic compensation algorithm. Both methods significantly enhance measurement accuracy and consistency within the biologically relevant 400-1200 Pa range (typical for adherent cancer cells). The compensation algorithm achieves a relative error below 5.2%. This work establishes a robust framework for cellular mechanical characterization, paving the way for incorporating greater biological complexity in future models.