Peer-reviewed veterinary case report
Ultrasound and peptides boost blood vessel growth after dog heart
By Cui, Jingjing et al.·Published in BioMed research international·2017·Department of Ultrasound Imaging, China·View original on PubMed →
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Original publication title: Enhancement of Angiogenesis by Ultrasound-Targeted Microbubble Destruction Combined with Nuclear Localization Signaling Peptides in Canine Myocardial Infarction.
- Species:
- dog
Plain-English summary
A group of dogs with heart attacks (myocardial infarction) underwent a new treatment using ultrasound and microbubbles to help improve blood vessel growth in their hearts. The treatment involved injecting a specific gene that promotes blood vessel formation, and the best results were seen in dogs that received a combination of ultrasound and a special delivery method for the gene. These dogs showed a significant increase in blood vessel density and improved heart function without any rise in heart damage markers. This approach appears to be both effective and safe for enhancing heart recovery in dogs.
People also search for: dog heart attack treatment · canine myocardial infarction therapy · improving dog heart function · ultrasound gene therapy for dogs
Abstract
OBJECTIVE: This study aimed to develop a gene delivery system using ultrasound-targeted microbubbles destruction (UTMD) combined with nuclear localization signal (NLS) and investigate its efficacy and safety for therapeutic angiogenesis in canine myocardial infarction (MI) model. METHODS: Fifty MI dogs were randomly divided into 5 groups and transfected with Ang-1 gene plasmid: (i) group A: only injection of microbubbles and Ang-1 plasmid; (ii) group B: only UTMD mediated gene transfection; (iii) group C: UTMD combined with classical NLS mediated gene transfection; (iv) group D: UTMD combined with mutational NLS mediated transfection; and (v) group E: UTMD combined with classical NLS in the presence of a nucleus transport blocker. The mRNA and protein expression of Ang-1 gene, microvessel density (MVD) cardiac troponin I (cTnI), and cardiac function were determined after transfection. RESULTS: The expression of mRNA and protein of Ang-1 gene in group C was significantly higher than that of the other groups (all< 0.01). The MVD of group C was 10.2-fold of group A and 8.1-fold of group E (< 0.01). The cardiac function in group C was significant improvement without cTnI rising. CONCLUSIONS: The gene delivery system composed of UTMD and NLS is efficient and safe.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/29259991/