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Peer-reviewed veterinary case report

Environmental air sampling to detect exotic Newcastle disease virus in two California commercial poultry flocks.

Journal:
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Year:
2005
Authors:
Hietala, Sharon K et al.
Affiliation:
University of California · United States
Species:
bird

Abstract

The 2002--2003 Exotic Newcastle Disease (END) outbreak in Southern California poultry provided an opportunity to evaluate environmental air sampling as an efficient and cost-effective means of sampling flocks for detection of a circulating virus. Exotic Newcastle Disease virus was detected by real-time reverse transcriptase PCR from air samples collected using a wetted-wall cyclone-style air sampler placed within 2 m of birds in 2 commercial flocks suspected of being naturally exposed to END virus during the outbreak. Exotic Newcastle Disease virus was detected after 2 hours of air sampling the poultry-house environments of the 2 naturally infected flocks.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/15825506/