Peer-reviewed veterinary case report
Erg mediates downregulation of claudin-5 in the brain endothelium of a murine experimental model of cerebral malaria.
- Journal:
- FEBS letters
- Year:
- 2019
- Authors:
- Liu, Fuhong et al.
- Affiliation:
- Shandong Provincial Qianfoshan Hospital · China
- Species:
- rodent
Abstract
Cerebral malaria (CM) is a severe complication with brain vascular hyperpermeability. Claudin-5 is the major component of tight junctions. To investigate the expression of claudin-5 in CM, we established a murine experimental cerebral malaria (ECM) model and an in vitro model by treating murine brain endothelial cells (bEnd3) with plasma from ECM mice. Expression of claudin-5 and the ETS transcription factor Erg was reduced in the brain endothelium of ECM mice. In bEnd3 cells exposed to ECM plasma, decreased expression of claudin-5 and Erg, and increased permeability were observed. Silencing of Erg significantly reduced Cldn5 expression. ChIP assays indicated that Erg binds to the -813 ETS motif of the murine Cldn5 gene promoter, and the binding is decreased by treatment with ECM plasma.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/31271645/