Peer-reviewed veterinary case report
Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection.
- Journal:
- STAR protocols
- Year:
- 2023
- Authors:
- Labora, Amanda et al.
- Affiliation:
- Department of Surgery · United States
Abstract
We report a technique to generate a murine model of lung metastases by selectively injecting tumor cells into the right heart ventricle under ultrasound guidance. First, we describe cell preparation and reference animal preparation as previously described. We then detail the technique using a previously described 3D-printed instrument stabilization device. Finally, we describe tumor growth surveillance using bioluminescent imaging. For complete details on the use and execution of this protocol, please refer to Labora et al..
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/37925631/