Peer-reviewed veterinary case report
Establishment and application of a multiplex PCR for rapid and simultaneous detection of six viruses in swine.
- Journal:
- Journal of virological methods
- Year:
- 2014
- Authors:
- Zeng, Zhiyong et al.
- Affiliation:
- College of Animal Sciences · China
Plain-English summary
Researchers have developed a new test called multiplex PCR that can quickly check for six different viruses in pigs at the same time. This test looks for three DNA viruses and three RNA viruses that commonly affect swine. It has been shown to be very sensitive, meaning it can detect even small amounts of the viruses in samples taken from sick pigs. The test is useful for diagnosing mixed infections, helping veterinarians identify what might be making the pigs ill. Overall, this new method is a promising tool for better diagnosing viral infections in pigs.
Abstract
A multiplex PCR assay was developed and evaluated subsequently for its effectiveness in simultaneously detecting mixed viral infections of swine. Specific primers were designed and used for testing the six swine viruses: three DNA viruses, including pseudorabies virus (PRV), porcine parvovirus (PPV), and porcine circovirus type 2 (PCV2); three common RNA viruses, including porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV), and Japanese encephalitis virus (JEV). This technique has shown to be highly sensitive in that the minimum detection amounts of nucleic acids from PRV, PPV, PCV2, PRRSV, CSFV, and JEV were 6.6, 96, 12.9, 10.5, 51, and 46 pg, respectively. It also was effective for detecting one or multiple viruses in the specimens, such as the lungs, spleens, lymph nodes, and tonsils collected from clinically ill pigs. The multiplex PCR method can detect simultaneously not only infection of the six viruses, but also other swine DNA and RNA viruses. Given its rapidity, specificity, and sensitivity, the multiplex PCR is a useful tool for diagnosing clinically the mixed infections of swine DNA and RNA viruses.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25116201/