Establishment of Animal Infection Model ofand Identification ofby Enzyme-Linked Immunosorbent Assay.

Abstract

is a crucial zoonotic parasite. Its larvae are more harmful than adult worms due to their ability to migrate through the host's tissues and organs. Therefore, it is necessary to establish an animal model of spargana for observing pathological changes and exploring diagnostic techniques.In this study, we infected Kunming mice and cats without any pathogens by feeding sparganum (with the scolex and neck) in order to understand the infection cycle ofand explore the preservation host of sparganosis. The infection ofwas determined by fecal detection and enzyme-linked immunosorbent assay (ELISA).In the model of cats, the eggs ofwere found in the feces ten days after the infection. The serum-specific IgG antibodies againstwere positive in experimental groups (mice and cats), and after sixty days, the S. mansoni worms isolated from experimental groups were collected.In conclusion, the experimental results show that mice and cats can be stably infected with S. mansoni through feeding sparganum (with the scolex and neck). The infection method of this study has the potential to establish a practical model for investigating the diagnostic process of, laying the groundwork for application and development. ELISA was used to diagnose mice and cats infected with sparganosis mansoni, providing a case for non-invasive identification of animal sparganosis.