Peer-reviewed veterinary case report
Evaluating the stability ofin various media under simulated environmental conditions of shipment for RT-qPCR assays.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2025
- Authors:
- Boggan, SaraBeth et al.
- Affiliation:
- Texas Tech School of Veterinary Medicine · United States
Abstract
is a notable reproductive pathogen in cattle, and the processes of sample collection, handling, transportation, and testing present considerable challenges for surveillance initiatives. Advancements include the development of a direct detection method forusing reverse-transcription quantitative real-time PCR. We compared the stability of samples in 3 different media: PBS, sterile saline, and lactated Ringer solution over periods of 24-120 h, with storage temperatures of 0°C, 20°C, 37°C, and 54.5°C to mimic shipping of samples in various environmental temperatures.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/40237409/