Peer-reviewed veterinary case report
How to test for H5 influenza in ducks?
By Lebarbenchon, Camille et al.·Published in Influenza and other respiratory viruses·2013·Department of Population Health, United States·View original on PubMed →
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Original publication title: Evaluation of a commercial enzyme-linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl.
- Species:
- bird
Plain-English summary
Researchers tested a specific blood test called the ID Screen Influenza H5 Antibody Competition enzyme-linked immunosorbent assay to see if it could detect antibodies against the H5 subtype of the influenza A virus in waterfowl, specifically Mallards and Pekin Ducks. The ducks were infected with different strains of the virus, both low and highly pathogenic types. They looked at how well the test worked after different waiting times and at different sample strengths. The results showed that all ducks infected with the low pathogenic strains had positive test results, but the test was better at detecting antibodies from the highly pathogenic strains. Overall, the test was effective in identifying the presence of antibodies in the infected ducks.
Abstract
The ID Screen Influenza H5 Antibody Competition enzyme-linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5-infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/24192340/