Peer-reviewed veterinary case report
Rapid canine C-reactive protein test accuracy compared to lab methods
By Hindenberg, Sarah et al.·Published in BMC veterinary research·2018·Department of Veterinary Clinical Sciences, Germany·View original on PubMed →
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Original publication title: Evaluation of a novel quantitative canine species-specific point-of-care assay for C-reactive protein.
- Species:
- dog
Plain-English summary
A new test for measuring C-reactive protein (CRP) in dogs was developed to help vets quickly assess inflammation levels. This test can provide results using a small in-house analyzer, making it easier for veterinarians to make decisions about a dog's health. The study showed that this new test works well and gives results that are similar to established methods. However, it’s important for vets to use the same test for follow-up checks to ensure accuracy. Overall, this test could help in diagnosing and monitoring conditions related to inflammation in dogs.
People also search for: dog inflammation test · C-reactive protein test for dogs · how to check dog CRP levels
Abstract
BACKGROUND: Species-specific point-of-care tests (POCT) permit a rapid analysis of canine C-reactive protein (CRP), enabling veterinarians to include CRP in clinical decisions. Aim of the study was to evaluate a novel POCT for canine CRP (Point Strip™ Canine CRP Assay) run on a small in-house-analyzer (Point Reader™ V) using lithium heparin plasma and to compare assay performance to an already established canine CRP assay (Gentian Canine CRP Immunoassay) run on two different bench top analyzers serving as reference methods (ABX Pentra 400, AU 5800). Linearity was assessed by stepwise dilution of plasma samples with high CRP concentrations. Limit of quantification (LoQ) was determined by repeated measurements of samples with low CRP concentrations. Coefficient of variation (CV) at low (10-50 mg/l), moderate (50-100 mg/l), and high (100-200 mg/l) CRP concentrations was investigated as well as possible interferences. Method comparison study was performed using 45 samples of healthy and diseased dogs. Quality criteria were fulfilled if the total observed error (TE = 2CV% + bias%) was below the minimal total allowable error of 44.4% (TE). Additionally, a reference range (n = 60 healthy dogs) was established. RESULTS: Linearity was present at CRP concentrations of 10-132 mg/l (≙ 361 mg/l CRP with reference method) with a LoQ set at 10 mg/l. At moderate to high CRP concentrations, intra- and inter-assay CVs were ≤ 8% and ≤ 11% respectively, while CVs ≤ 22% and ≤ 28% were present at low concentrations. No interferences were observed at concentrations of 4 g/l hemoglobin, 800 mg/l bilirubin and 8 g/l triglycerides. Method comparison study demonstrated an excellent correlation with both reference methods (r = 0.98 for ABX Pentra 400; 0.99 for AU 5800), though revealing a proportional bias of 19.7% (ABX Pentra 400) and 10.7% (AU 5800) respectively. TEwas 26.7-31.9% and 16.7-21.9% and thus < TE. Healthy dogs presented with CRP values ≤11.9 mg/l. CONCLUSIONS: The POCT precisely detects canine CRP at clinically relevant moderate and high CRP concentrations. The assay correlates well with both reference methods. Due to the bias, however, follow-up examinations should be performed with the same assay and analyzer.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/29554960/