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Peer-reviewed veterinary case report

Evaluation of a real-time polymerase chain reaction assay of the outer membrane protein P2 gene for the detection of Haemophilus parasuis in clinical samples.

Journal:
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire
Year:
2014
Authors:
McDowall, Rebeccah et al.
Affiliation:
University of Guelph · Canada

Abstract

A real-time polymerase chain reaction (PCR) assay of the outer membrane protein (OMP) P2 gene was developed and used to test 97 putative Haemophilus parasuis pure cultures and 175 clinical tissue samples. With standard culture isolation as the gold standard, the diagnostic sensitivity and specificity of the PCR assay were determined to be 83% and 80%, respectively.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/24688178/