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Peer-reviewed veterinary case report

Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016.

Journal:
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
Year:
2017
Authors:
Larsen, Jesper et al.
Affiliation:
Statens Serum Institut

Abstract

We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/28749335/