Peer-reviewed veterinary case report
Testing for Histoplasma antibodies in cats and dogs
By Tims, Rebecca et al.Ā·Published in Journal of veterinary internal medicineĀ·2023Ā·Veterinary Specialists of North Texas, United StatesĀ·View original on PubMed ā
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Original publication title: Evaluation of an enzyme immunoassay and immunodiffusion for detection of anti-Histoplasma antibodies in serum from cats and dogs.
Plain-English summary
A group of 37 cats and 22 dogs with suspected histoplasmosis (a fungal infection) were tested for antibodies to see if they had the disease. The tests showed that an enzyme immunoassay (EIA) was effective, detecting antibodies in 81% of cats and 77% of dogs, while the older immunodiffusion test failed to identify any cases in cats and only a few in dogs. This means that the EIA test is a reliable option for diagnosing histoplasmosis in pets, while the immunodiffusion test is not recommended due to its poor performance.
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Abstract
BACKGROUND: Histoplasma antigen and anti-Histoplasma antibody detection are used to support the diagnosis of histoplasmosis. There is a paucity of published data on antibody assays. OBJECTIVES: Our primary hypothesis was that anti-Histoplasma immunoglobulin G (IgG) antibody detection using enzyme immunoassay (EIA) will be more sensitive as compared to immunodiffusion (ID). ANIMALS: Thirty-seven cats and 22 dogs with proven or probable histoplasmosis; 157 negative control animals. METHODS: Residual stored sera were tested for anti-Histoplasma antibodies using EIA and ID. Results of urine antigen EIA were reviewed retrospectively. Diagnostic sensitivity was calculated for all three assays and compared between immunoglobulin G (IgG) EIA and ID. The diagnostic sensitivity of urine antigen EIA and IgG EIA, interpreted in parallel, was reported. RESULTS: Sensitivity of IgG EIA was 30/37 (81.1%; 95% confidence interval [CI], 68.5%-93.4%) in cats and 17/22 (77.3%; 95% CI, 59.8%-94.8%) in dogs. Diagnostic sensitivity of ID was 0/37 (0%; 95% CI, 0%-9.5%) in cats and 3/22 (13.6%; 95% CI, 0%-28.0%) in dogs. Immunoglobulin G EIA was positive in all animals (2 cats and 2 dogs) with histoplasmosis but without detectable antigen in urine. Diagnostic specificity of IgG EIA was 18/19 (94.7%; 95% CI, 74.0%-99.9%) in cats and 128/138 (92.8%; 95% CI, 87.1%-96.5%) in dogs. CONCLUSION AND CLINICAL IMPORTANCE: Antibody detection by EIA can be used to support the diagnosis of histoplasmosis in cats and dogs. Immunodiffusion has an unacceptably low diagnostic sensitivity and is not recommended.
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Search related cases āOriginal publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/37102374/