Peer-reviewed veterinary case report
Evaluation of Babesia bigemina 200 kDa recombinant antigen in enzyme-linked immunosorbent assay.
- Journal:
- Parasitology research
- Year:
- 2009
- Authors:
- Altangerel, Khukhuu et al.
- Affiliation:
- Obihiro University of Agriculture and Veterinary Medicine · Japan
Abstract
A truncated fragment of the gene encoding the 200-kDa protein (P200) of Babesia bigemina was cloned into a plasmid vector, pGEX-4 T-1 and expressed in Escherichia coli as a glutathione-S-transferase fused protein. An indirect enzyme-linked immunosorbent assay (ELISA) using the rp200/CT detected specific antibodies in cattle experimentally infected with B. bigemina. Furthermore, the antigen did not cross-react with antibodies to Babesia bovis, a closely related Babesia parasite indicating that rp200/CT is a specific antigen for the diagnosis of B. bigemina infection. Additionally, ELISA using p200/CT and polymerase chain reaction were conducted on serum and corresponding DNA samples obtained from field cattle to evaluate the diagnostic utility of the p200/CT antigen. Results from the current study suggest that p200/CT ELISA is a sensitive and specific method for improved serodiagnosis of B. bigemina infection.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/19294421/