Peer-reviewed veterinary case report
Antiretinal antibodies found in dogs with sudden retinal degeneration
By Keller, Rachel L et al.·Published in Veterinary ophthalmology·2006·Department of Small Animal Clinical Sciences, United States·View original on PubMed →
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Original publication title: Evaluation of canine serum for the presence of antiretinal autoantibodies in sudden acquired retinal degeneration syndrome.
- Species:
- dog
Plain-English summary
A group of dogs with sudden vision loss, known as sudden acquired retinal degeneration syndrome (SARDS), were tested for specific antibodies in their blood that might indicate an immune response affecting their eyes. Researchers compared the blood samples from these dogs to those from healthy dogs. The tests showed that there were no antibodies linked to eye problems in the dogs with SARDS, suggesting that their vision loss may not be due to an autoimmune issue. This information can help veterinarians better understand and manage SARDS in dogs.
People also search for: dog sudden vision loss · SARDS in dogs · treatment for dog eye problems
Abstract
OBJECTIVE: To determine the presence of serum antiretinal antibodies in sudden acquired retinal degeneration syndrome (SARDS) affected dogs and the size of the antigen to which these antibodies bind via the use of enzyme-linked immunosorbent assay (ELISA) and Western blot immunoassays. ANIMALS STUDIED: Serum was collected from 13 dogs affected by SARDS and five dogs with normal ocular examinations. PROCEDURES: All serum samples were subjected to ELISA with saline-soluble canine retinal tissue and Western blot analyses with SDS solubilized normal canine retinal tissue as the antigen. Antirecoverin (23 kDa) and antiheat shock cognate (65 kDa) antibodies were used as positive controls for both procedures. Affinity-purified goat antidog IgG and IgM labeled with horseradish peroxidase were used for all clinical samples and goat antirabbit IgG was used as the secondary antibody for the positive controls. RESULTS: ELISA demonstrated antibody reaction with all samples. Western blot immunoassays identified multiple bands in all canine serum samples, as well as in negative controls. Approximate sizes of the bands were 25 and 50 kDa, corresponding to IgG light and heavy chains, respectively. CONCLUSION: No antiretinal autoantibodies were identified in the serum of dogs affected by SARDS as compared to normal canine patients.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/16634935/