Peer-reviewed veterinary case report
New chicken antibody tests to detect canine parvovirus in dog poop
By He, Jinxin et al.·Published in Viral immunology·2015·1 College of Veterinary Medicine, China·View original on PubMed →
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Original publication title: Evaluation of Chicken IgY Generated Against Canine Parvovirus Viral-Like Particles and Development of Enzyme-Linked Immunosorbent Assay and Immunochromatographic Assay for Canine Parvovirus Detection.
- Species:
- dog
Plain-English summary
A study developed new tests to detect canine parvovirus (CPV) in dogs showing diarrhea. Researchers created antibodies from chicken eggs that can identify CPV in dog feces. They tested 34 fecal samples from dogs with diarrhea and found that about 26% were positive for CPV using a standard test, while the new tests showed similar results with high accuracy. These new tests could help veterinarians quickly diagnose CPV in dogs, which is crucial for effective treatment.
People also search for: dog diarrhea parvovirus test · how to test for canine parvovirus · symptoms of parvovirus in dogs
Abstract
Immunoglobulin Y (IgY) antibodies were generated against canine parvovirus virus-like particles (CPV-VLPs) antigen using chickens. Anti-CPV-VLPs-IgY was extracted from hen egg yolk and used for developing enzyme-linked immunosorbent assay (ELISA) and immunochromatographic assay (ICA) for the detection of CPV in dog feces. The cutoff negative values for anti-CPV-VLPs-IgY were determined using negative fecal samples (already confirmed by polymerase chain reaction [PCR]). In both ELISA and ICA, there was no cross-reaction with other diarrheal pathogens. Thirty-four fecal samples were collected from dogs with diarrhea, of which 26.47% were confirmed as CPV-positive samples by PCR, while 29.41% and 32.35% of the samples were found to be positive by ELISA and ICA, respectively. The developed ELISA and ICA exhibited 97.06% and 94.12% conformity with PCR. Higher sensitivity and specificity were observed for IgY-based ELISA and ICA. Thus, they could be suitable for routine use in the diagnosis of CPV in dogs.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/26469376/