Peer-reviewed veterinary case report
New ELISA test detects distemper infection in dogs using nucleocapsid
By Latha, D et al.·Published in Veterinary microbiology·2007·Anna University, India·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Evaluation of ELISA based on the conserved and functional middle region of nucleocapsid protein to detect distemper infection in dogs.
- Species:
- dog
Plain-English summary
A group of dogs with distemper were tested for the virus using a new blood test based on a specific protein from the virus. The test showed a strong correlation with traditional methods, meaning it was effective in detecting the infection. Both infected and vaccinated dogs had antibodies against the virus, confirming the test's reliability. This new test could help veterinarians diagnose canine distemper more accurately and quickly, improving treatment options for affected dogs.
People also search for: dog distemper symptoms · how to test for distemper in dogs · canine distemper treatment options
Abstract
A 287bp fragment from the middle region of the nucleocapsid protein of canine distemper virus (CDV) was amplified from the conjunctival samples of distemper-infected dogs and was cloned into pRSET B vector. The recombinant protein was expressed as a 16-kDa-fusion protein with histidine tag in E. coli. Sera of distemper-infected and vaccinated dogs contained IgG antibodies against the purified recombinant protein as observed by enzyme linked immunosorbent assays (ELISA) and showed a strong correlation (r=0.882, p<0.0001 at 95% CI) and good agreement (kappa=0.718) with the conventional tissue culture viral antigen based ELISA. Further, the results of recombinant protein based ELISA and Western blotting with the sera from the infected and vaccinated dogs correlated well (kappa=0.8226). These findings recommend the use of the recombinant protein in the serodiagnosis of canine distemper virus infection in dogs.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/17224247/